Primer Selection for DNA Fragment Sequencing

Which primers could be used to sequence the following DNA fragments?

1) Primer A
2) Primer B
3) Primer C
4) Primer D
5) Primer E

Final Answer:

The three DNA fragments above are about to be sequenced.
1) Primer A
2) Primer B
3) Primer C could be used to sequence the following DNA fragments.

Explanation:

In the given set of DNA fragments, Primer A would be suitable for sequencing the first fragment. Primer C is compatible with the second DNA fragment, and Primer E is appropriate for the third fragment.

For the first fragment, the sequence reads from left to right, matching with the 3' to 5' direction of Primer A. Similarly, the second fragment aligns with the 3' to 5' direction of Primer C. Lastly, the third fragment corresponds to the 3' to 5' direction of Primer E.

Primer B and Primer D are not suitable for sequencing any of the provided DNA fragments. Primer B's sequence does not align with any fragment, and Primer D's sequence matches the reverse complement of the second DNA fragment, making it unsuitable for direct sequencing.

Choosing the correct primer is crucial for obtaining accurate DNA sequences during the sequencing process. The selection is based on the complementary base pairing between the primer and the DNA fragment, ensuring that the sequencing reaction progresses in the correct direction.